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FERTIUP® and CARD MEDIUM

Pups obtained via in vitro fertilization
FERTIUP® Mouse Cryoprotectant guards against damage to spermatozoa during cryopreservation.
FERTIUP® Mouse Preincubation Medium significantly increases the fertilizing ability of spermatozoa.
CARD MEDIUM works on the zona pellucida of oocytes to make them easier to fertilize.
Examples of Use

Mouse strains can be successfully archived using
FERTIUP® Mouse Cryoprotectant
to cryopreserve sperm in lieu of live mice.
This allows us to make massive savings in cost and space required for
breeding management.

When live mice of a certain strain are required,
the cryopreserved spermatozoa are thawed, treated with
FERTIUP® Mouse Preincubation Medium and CARD MEDIUM to
increase their fertility, and used to fertilize oocytes.
The embryos obtained from fertilization can then be transferred
into recipient parents to produce the mice required.


 

Even strains which are difficult to obtain via natural crossbreeding
can be successfully produced in a short period of time,
by treating fresh spermatozoa taken from a male mouse with
FERTIUP® Mouse Preincubation Medium and CARD MEDIUM Medium,
using the spermatozoa for in vitro fertilization,
then transferring the embryos obtained into recipient parents.


 

It is also possible to produce mouse pups without
the presence of a male mouse
by using just the cauda epididymis of a male mouse,
which has been sent safely and cheaply using
CARD cold temperature transport kit
The spermatozoa taken from the cauda epididymis are preincubated in
ERTIUP® Mouse Sperm Preincubation Medium before being used for
in vitro fertilization using CARD MEDIUM.
The embryos obtained from fertilization can then be transferred into recipient parents.

 

High fertilization rates can be obtained even in strains such as 129 and BALB/c,
which are known for their low in vitro fertilization rates, by preincubating spermatozoa in
FERTIUP® Mouse Preincubation Medium and
CARD MEDIUMnd carrying out in vitro fertilization in CARD MEDIUM.