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Reagents and equipment for use in rat reproductive engineering

About reagents
As a result of joint research carried out by ourselves and Kumamoto University, we have developed a number of reagents for use in rat reproductive engineering.
CARD FERTIUP®Rat Sperm Cryopreservation Agent
CARD FERTIUP® Rat Sperm Cryopreservation Agent is a newly-developed agent which enables researchers to efficiently carry out cryopreservation of rat sperm, which until now has been notoriously difficult to cryopreserve. Conventional rat sperm cryopreservation methods are unable to effectively maintain the motility of frozen-thawed sperm. On the other hand, rat sperm cryopreservation carried out using CARD FERTIUP🄬 Rat Sperm Cryopreservation Agent yields superior motility even in frozen-thawed rat sperm, allowing for the production of many rat pups via IVF and embryo transfer.
CARD Rat IVF medium
CARD Rat IVF Medium is a new IVF medium developed specially for use in rat IVF.
CARD Rat Sperm Freezing kit
CARD Rat Sperm Freezing Kit is a kit that facilitates the effective production of high-quality frozen rat sperm during rat sperm cryopreservation using CARD FERTIUP® Rat Sperm Cryopreservation Agent. With this kit, researchers can appropriately carry out the rat sperm cooling process during rat sperm cryopreservation.

Characteristics

・Enables IVF to be carried out using frozen-thawed rat sperm.
・Contributes to a reduction in breeding costs through improvements in rat strain preservation efficiency.
・Facilitates the timely acquisition of live rats when they are required for experiments through application
 to genetically modified rats

Product structure

 Product name standard 
 CARD FERTIUP®Rat Sperm Cryopreservation Agent 1.5 mL   
 CARD Rat IVF medium  1.0 mL   
 CARD Rat sperm freezing kit Kit   
Components : Styrofoam box, straw holding support.  
 
【CARD Rat sperm freezing kit : Components】

Reference data

Fig. IVF fertilisation rates using fresh and frozen-thawed rat sperm.


Chart. Rate of development of fertilised oocytes derived from frozen-thawed rat sperm to the blastocyst stage.
Sperm No. No. of 2-cell embryos No. of blastocysts Developmental rate(%)
Fresh 1 100 58 58
2 100 63 63
3 100 65 65
4 100 71 71
Total 400 257 64.3±5.4
Frozen 1 100 72 72
2 100 57 57
3 100 64 64
4 100 53 53
Total 400 246 61.5±5.4
Citation source:
Joint Research Field Division of Reproductive Biotechnology and Innovation, IRDA in Kumamoto University
Division of Reproductive Engineering, IRDA in Kumamoto University
    Introducing reagents for use in rat reproductive engineering (PDF)
    Introducing CARD FERTIUP® Rat Sperm Cryopreservation Agent (PDF)