九動株式会社

As a result of joint research carried out by ourselves and Kumamoto University, we have developed a number of reagents for use in rat reproductive engineering.

CARD HyperOva for RAT（Superovulation Reagent for Rat）

CARD HyperOva for RAT is a superovulation reagent that induces ovulation of rat at higher efficiency than the conventional method (PMSG-hCG method).

CARD FERTIUP Rat Sperm Cryopreservation Agent

CARD FERTIUP Rat Sperm Cryopreservation Agent is a newly-developed agent which enables researchers to efficiently carry out cryopreservation of rat sperm, which until now has been notoriously difficult to cryopreserve. Conventional rat sperm cryopreservation methods are unable to effectively maintain the motility of frozen-thawed sperm. On the other hand, rat sperm cryopreservation carried out using CARD FERTIUP Rat Sperm Cryopreservation Agent yields superior motility even in frozen-thawed rat sperm, allowing for the production of many rat pups via IVF and embryo transfer.

CARD Rat IVF medium

CARD Rat IVF Medium is a new IVF medium developed specially for use in rat IVF.

CARD Rat Sperm Freezing kit

CARD Rat Sperm Freezing Kit is a kit that facilitates the effective production of high-quality frozen rat sperm during rat sperm cryopreservation using CARD FERTIUP Rat Sperm Cryopreservation Agent. With this kit, researchers can appropriately carry out the rat sperm cooling process during rat sperm cryopreservation.

Characteristics

Enables us to obtain oocytes more effectively from rats.
Expected to reduce the number of female rats for IVF.(Contribution to the Reduction of 3Rs)
Enables IVF to be carried out using frozen-thawed rat sperm.
Contributes to a reduction in breeding costs through improvements in rat strain preservation efficiency.
Facilitates the timely acquisition of live rats when they are required for experiments through application to genetically modified rats

Product structure

Product name	standard
CARD HyperOva for RAT	1.2 mL
CARD FERTIU Rat Sperm Cryopreservation Agent	1.5 mL
CARD Rat IVF medium	1.0 mL
CARD Rat sperm freezing kit	Kit
CARD Rat sperm freezing kit	Components : Styrofoam box, straw holding support.
【CARD Rat sperm freezing kit :　Components】

Reference data

Fig. Effect of CARD HyperOva for RAT

Fig. IVF fertilisation rates using fresh and frozen-thawed rat sperm.

Chart. Rate of development of fertilised oocytes derived from frozen-thawed rat sperm to the blastocyst stage.

Sperm	No.	No. of 2-cell embryos	No. of blastocysts	Developmental rate(%)
Fresh	1	100	58	58
	2	100	63	63
	3	100	65	65
	4	100	71	71
	Total	400	257	64.3±5.4
Frozen	1	100	72	72
	2	100	57	57
	3	100	64	64
	4	100	53	53
	Total	400	246	61.5±5.4