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生殖工学試薬

References

Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, KI., Irie, T. and Nakagata, N. Methyl-beta-cyclodextrin improves the fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Biol Reprod. 78(3):546-51. 2008.


Takeo, T and Nakagata, N. Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm. Lab Anim. 44(2):132-7. 2010


Nakagata N. 2011. Cryopreservation of mouse spermatozoa and in vitro fertilization. Methods Mol Biol. 693: 57-734


Takeo, T and Nakagata, N. Reduced glutathione enhances fertility of frozen/thawed C57BL/6 mouse sperm after exposure to methyl-beta-cyclodextrin. Biol Reprod. 85(5):1066-72, 2011.